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KMID : 0357319920270030215
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Journal of the Korean Society for Microbiology
1992 Volume.27 No. 3 p.215 ~ p.230
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Production of and Sensitivity to Bacteriocin, "Vulnificin" of Vibrio Vulnificus
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Abstract
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Vibrio vulnificus, a halophilic vibrio is an estuarine bacterium that has been associated with severe and frequently fatal wound infections and life-thretening septicemia, particulary in immunocompromised individuals or in patients with
pre-existing
hepatic disease. For epidemiological studies, biobyping, serotyping, bacteriophage typing and comparison of antibiograms were widly employed. Bacteriocin are defined as antibacterial substance produced by various species of bacteria which are
active
usually against closely related organisms. Bacteriocins were demonstrated in a variety of bacteria and bacteriocins have found widespread application in epidemiological studies as specific markers of bacteria. It was proposed by T.Y. Ha et al.,
to
give
bacteriocins produced by B Vibrio vulnificus "vulnificins". In the present study, 24 clinical isolates of V. vulnificus, 15 isolates from oysters and 8 well-documented laboratory strains were subjected to screen on potential producers and
potential
indicators of vulnificin, applying ultraduction method. Sensitivity of each several strains of Serratia marcescesn, Pseudomonas aeruginosa, Shigella flxneri, Salmonella typhi and Yersinia enterocolitica to a variety of vulnificins produced by V.
vulnificus isolated strains were also carried out. In addition, the isolated strains of V. vulnificus were tested for antibvacterial susceptibility to 11 drugs by microdilution troth method for the possible correlationship of antibiotic and
vulnificin
resistance.
All the tested strains of V. vulnificus produced vulnificin active against one or more strains of 9 standard indicator strains used for vibriocin typing whgich were provided by Dr. S.G.D astida, India. Of 33 strains of V. vulnificus examined, 9
strains
(27.3%) produced vulnificins inhibiting 7 indicator strains, 6 strains (18.2%) prouced vulnificins inhibiting 5 indicators and the other strains produced vulnificins inhibiting 2, 3, 4, 6 or 8 indicators, respectively, and 32 different inhibition
patterns were observed, suggesting that the spectrum of vulnificin activity and the sensitive spectrum of indicator strains were broad. Out of 47 isolates of V. vulnificus, all were vulnificin producer strains producing vulnificin active against
6-37
strains of 47 indicators which were the isolates of V. vulnificus. Of 47 vulnificin producer strains, 8 strains(P, Q R, S, T, U, V and W strains) were found to be good producer strains producing vulnificins inhibting 10-28 indicator strains among
the 47
isolates, particulary producer strain P produced a vulnificin inhibiting 28 strains (59.6%). Of 47 isolates, particulary producer strain P produced a vulnificin inhibiting 28 strains (59.6%). Of 47 indicator strains, 10 strains(JI-J5 and K1-K5)
were
found to be good indicator strains sensitive to 16-33 vulnifins produced by the isolates, prticulary an indicator strains K4 was sensitive to vulnificins produced by 33 producer strains (70.2%), suggesting sensitive spectrum was consideratly
broad.
Interestingly, 24 strains (51.1%) of producer strains was susceptible to its own active principle, particulary 3 strains (6.9%) were highly susceptible. All 5 strains of S. marcescens were sensitive to vulnificins produced by 1-4 strains(s) of 8
good
producer strains and producer strains P and U produced vulnificins inhibiting 4 strains of S. marcesens, respectively. Of 5 strains of P. aeruginosa, 4 strains were sensitive to 3-7 vulnificins and 1 strain was sensitive to 7 vulnificins, but
other
one
strain was resistant to the vulnificins, Each strain of Shigella and Salmonella tested were sensitive to 1-6 vulnificin(s) and producer strains P produced vuljhificin inhibitingnone of Shigella strains. Three strains of Y. enterocolitica teseted
were
sensitive to vulnificins, and interestingly, one of the strains were sensitive to all individual velnificins produced by good vulnificin produced by good vulnificin producer strains, suggesting this particular strains may be used as good
indicators
strains for the detection of vulnificins production. Out of 47 isolates of V. vulmficus, none strains was resistant to drugs such chloramphenicol, tetracycline, cephalothin or moxalactam. Among drug resistant strains, the strains resistant to
streptomycin (SM) were encountered most frequently with the rate of 36.2% (17 strains) and SM-resistant strains seemed to be especially resistant to vulnificin produced by one strains. Taken together, it was found in the present study that all
the
isolates of V. vulnificus produced vulnificins inhibiting one or more standard indicator strains and the other also detected both 8 goo producerws of vulnificins which has the broad activity spectrum against the isolates and 10 good indicators
sensitive
to a variety of vulnificins, and this strongly surggest these good producers and indicators may be used efficiently for establishing bacteriocin typing shcheme and for the detection of bacteriocinogeny and sensitivity in V. vulnificus.
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KEYWORD
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